UNIVERSITY PARK, Pa. — The nearly century-old effort to employ selective breeding to rescue the American chestnut, which has been rendered functionally extinct by an introduced disease, eventually will succeed, but it will take longer than many people expect.
That’s the gist of findings from a new study conducted by a research team composed of scientists from Penn State, the American Chestnut Foundation and State University of New York.
The research should tamp down expectations of both the public and some members of the science community that victory is imminent, but it also provides reassurance that the rescue ultimately will result in chestnuts flourishing in forests again, according to the lead author, Kim Steiner, professor of forest biology, Penn State College of Agricultural Sciences.
Reviewing records
To reach their conclusions, researchers reviewed and evaluated decades of breeding records and transgenic experiments, new experimental data, and made projections related to how recurrent selection and incorporation of transgenic material into breeding lines will expedite blight resistance.
They considered experimentally-based estimates of heritability and genetic gain for blight resistance that were never available before this research was conducted.
“Those estimates are why we know, now, for sure that it is just a matter of time,” Steiner said.
“Very few people understand the magnitude of the breeding challenge embarked upon by The American Chestnut Foundation when it began in 1983. Just to complete the B3F2 generation of breeding and selection — the final generation as originally envisioned — has meant that 73,000 trees must be created by hand pollination and grown and tested in plantations for a minimum of three years.”
Breeding program
The process began in 2002 with the foundation’s main breeding program and probably will not be completed until 2022, Steiner added.
Furthermore, it is being duplicated through the work of volunteers in 13 affiliated state programs. The Pennsylvania program is overseen by Sara Fitzsimmons, a research technician in Penn State’s Department of Ecosystem Science and Management and one of the study’s co-authors.
Developing an accurate and comprehensive status report on the chestnut-rescue effort and identifying next steps and expectations of progress were the main reasons behind the research, explained Steiner, who is a board member of The American Chestnut Foundation and senior science advisor to the board
“For a number of years, the foundation has made seed from B3F2 trees available for special purposes,” he said. “But the average blight resistance of those seedlings was not expected to be high until the B3F2 plantation is completed and culled to the few hundred most resistant trees. Our current findings indicate that, even then, average levels of resistance will not match Chinese chestnut.”
High resistance
However, some seeds from the B3F2 plantation will carry genes for high resistance, and there may be enough of them to begin restoration work, Steiner noted. Ultimately, he explained, the foundation’s goal is to give American chestnut the “genetic wherewithal” to survive to maturity and evolve on its own.
“So we are not concerned about uniformity from tree to tree in the way that a landscape nursery must be,” Steiner said.
In the research, which was published in New Forests in April, progress and future steps were summarized in two approaches: First, The American Chestnut Foundation’s system of hybridizing with the blight-resistant Chinese chestnut species and then backcrossing repeatedly to recover the American type tree.
Secondly, the State University of New York College of Environmental Science and Forestry’s transformation of American chestnut with a resistance-conferring transgene. That effort has been sponsored by the New York Chapter of The American Chestnut Foundation.
Providing evidence
Several decades of effort have been invested in each approach, and more work remains, researchers noted, but results provide the best experimental evidence since the blight (Cryphonectria parasitica) hit North America sometime after 1904 that success is within practical reach.
“We believe that the breeding program may need an additional one or two generations of recurrent selection to maximize resistance,” Steiner said. “The New York transgenic tree still must undergo regulatory review before release, and it must be tested in harshly competitive, natural environments before its true worth is understood. If it proves out, we expect that the New York work will be integrated with the foundation’s conventional breeding program because both lines of work have inherent strengths.”
Steiner, who is director of The Arboretum at Penn State, where the Pennsylvania program’s B3F2 plantation is growing, called the chestnut-restoration effort the most ambitious, protracted and technically difficult rescue of a species that ever has been undertaken.
